Using artificial miRNA to improve soybean resistance to nematodes;
Bin Yu ( University of Nebraska), Loren Giesler ( University of Nebraska), Tom Clemente ( University of Nebraska) ($60240). The Project Manager's email is firstname.lastname@example.org.
Key Words: Soybean Cyst Nematode - Genetic Resistance
The goal of this research is to develop soybean varieties with high resistance to SCN using artificial miRNA technology and to better understand the function of miRNA in soybean’s resistance to SCN. The specific aims are: • Generate SCN resistance varieties using miRNA technology. An artificial miRNA construct targeting a ribosomal gene that is essential for SCN survival will be made and over-expressed in soybean. The resistance of transgenic line to SCN will be examined. • Identify miRNAs regulating soybean resistance to SCN infection. Next-generation is a powerful approach for identifying miRNAs from a variety of species. In this project, they will apply this technology to identify soybean miRNAs involved in response to SCN treatment.
This project is funded by Nebraska Soybean Board