R-gene Clusters for Phytophthora Sojae resistance: Cloning Rps Genes;
Anne Dorrance ( The Ohio State University) ($198646). The Project Manager's email is firstname.lastname@example.org.
Key Words: Phytophthora sojae, Phytophthora Root Rot
Phytophthora sojae is one of the most prevalent soybean pathogens in the U.S.; however, it has been successfully managed with the deployment of resistance-gene (R-gene) mediated resistance in commercial varieties. All known P. sojae Rps genes map to complex loci containing multiple R-genes. Sequences from several of these R-gene rich regions are now available from several soybean genotypes (Williams82, PI 96983, PI 399073, and L76-1988) and more is in process from PI 399073. Sequencing approaches have identified numerous candidate R-genes, but the identification of the actual resistance genes remains difficult. Our goals are to sequence the complex loci containing Rps2, Rps3, and Rps8 from each of the resistant parents and use expression data to identify the expressed candidate resistance genes; and; secondly to use virus induced gene silencing (VIGS) to assay the function of these candidate resistance genes in response to P. sojae infection. By combining sequencing, expression and virus-induced gene silencing data we aim to identify the Rps2, Rps3 and Rps8 resistance genes.
This project is funded by United Soybean Board